A Carbamate-Type Cholinesterase Inhibitor 2-sec-Butylphenyl N-Methylcarbamate Insecticide Blocks L-Type Ca2+ Channel in Guinea Pig Ventricular Myocytes
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منابع مشابه
L-type and T-type Ca2+ current in cultured ventricular guinea pig myocytes.
The aim of this investigation was to study L-type and T-type Ca(2+) current (I(CaL) and I(CaT)) in short-term cultured adult guinea pig ventricular myocytes. The isolated myocytes were suspended in serum-supplemented medium up to 5 days. Using whole-cell patch clamp techniques ICaL and ICaT were studied by applying voltage protocols from different holding potentials (-40 and -90 mV). After 5 da...
متن کاملProperties of L-type calcium channel gating current in isolated guinea pig ventricular myocytes
Nonlinear capacitative current (charge movement) was compared to the Ca current (ICa) in single guinea pig ventricular myocytes. It was concluded that the charge movement seen with depolarizing test steps from -50 mV is dominated by L-type Ca channel gating current, because of the following observations. (a) Ca channel inactivation and the immobilization of the gating current had similar voltag...
متن کاملCalmodulin reverses rundown of L-type Ca(2+) channels in guinea pig ventricular myocytes.
Calmodulin (CaM) is implicated in regulation of Ca(2+) channels as a Ca(2+) sensor. The effect of CaM on rundown of L-type Ca(2+) channels in inside-out patch form was investigated in guinea pig ventricular myocytes. Ca(2+) channel activity disappeared within 1-3 min and did not reappear when the patch was excised and exposed to an artificial intracellular solution. However, application of CaM ...
متن کاملInhibition of L-type Ca(2+) current in Guinea pig ventricular myocytes by cisapride.
The effect of cisapride on L-type Ca(2+) current (I(Ca,L)) was studied in guinea pig ventricular myocytes using a whole-cell voltage-clamp technique and a conventional action potential recording method. Myocytes were held at -40 mV, and internally dialyzed and externally perfused with Na(+)- and K(+)-free solutions; cisapride elicited a concentration-dependent block of peak I(Ca,L), with a half...
متن کاملChanges in intracellular Ca2+concentration induced by L-type Ca2+ channel current in guinea pig gastric myocytes.
We investigated the relationship between voltage-operated Ca2+ channel current and the corresponding intracellular Ca2+concentration ([Ca2+]i) change (Ca2+ transient) in guinea pig gastric myocytes. Fluorescence microspectroscopy was combined with conventional whole cell patch-clamp technique, and fura 2 (80 μM) was added to CsCl-rich pipette solution. Step depolarization to 0 mV induced inward...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 2002
ISSN: 0021-5198
DOI: 10.1254/jjp.90.12